CRISPR 技术发展史思维导图

CRISPR locus described

"CRISPR"term first coined

Cas9 identified as a single-effector endonuclease

spacers transcribed as crRNAs identified

CRISPR/Cas9 identified as the adaptive immune system in prokaryotes

trRNA identified to form duplex with crRNA to guide Cas9

sgRNA construct developed for simplification

CRISPR/Cas9 gene editing achieved in mammalian cells

First CRISPR clinical trial for against HIV-1（China)

First CRISPR germline editing inhuman embryos(China)

First CRISPR clinical trial for cancer immunotherapy（USA)