GPR158 in pyramidal neurons modulates social novelty思维导图

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GPR158 in pyramidal neurons modulates social novelty思维导图

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GPR158 in pyramidal neurons modulates social novelty思维导图

锥体神经元中的GPR158调节社会新颖性介绍

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锥体神经元中突触后重新资本化测试

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GPR158 in pyramidal neurons modulates social novelty思维导图模板大纲

Social novelty deficit in KO mice. Behavioral tests with KO mice, to evaluate sociability, social novelty, stereotyped behaviors and cognitive ability

Deciding mPFC as the target area via c-Fos. mPFC, CA1, CA3, DG and VMH were investigated. mPFC: Higher expression, and larger difference

E-I imbalance: ELISA-Glutamate reduction

Presynaptic

TEM: Reduction of glutamate vesicles

子主题 1

Golgi: dendritic spines

Postsynaptic

Reduced amplitude and decay time of EPSC

Reduced expression of GluN2B and p-GluB2B

Mechanism verification

Recapitualate tests

CamK2aCre;Gpr158flox/flox mice

shRNA-Gpr158 into mPFC

Rescue tests

Gpr158 re-expression in mPFC with Lenti-virus

Chemogenetic enhancement of CamK2A+ neurons in mPFC

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